LiveBox1
LiveBox1 (LB1) is a transparent bioreactor, suitable to develop dynamic in-vitro models in attached cell culture conditions, maintaining the standard cell culture and monitoring protocols.
FAQ
Yes, LB1 is compatible with the cell growth area of a single slot of a -6, -12, -24 multiwell plate.
Yes, due to the transparency of the chamber. Moreover, LB1 is compatible with standard microscope stative, slide and plate readers.
Yes, you can recollect the biological material, removing the support (i.e., glass disc).
Yes, the chamber can be sterilized using autoclave 10 times.
Protocols
LiveBox2
LiveBox2 (LB2) is a transparent bioreactor, designed to mimic pato-physiological barriers in dynamic conditions, both in air-liquid (ALI) and liquid-liquid (LLI) interfaces.
FAQ
Yes, you can recollect the biological material, removing the support (i.e., membrane, glass disc).
Yes, LB2 is equipped with a porous and removable membrane, which is compatible in porosity and material with the standard transwell. Moreover, the chamber is compliant with the cell growth area of a transwell of a -6, -12, -24 multiwell plate.
Yes, the ALI module is an add-on which replaces the top glass slide, allowing for a direct access to the wet volume of the chamber (note: it’s compatible also with LB1).
Yes, the sigmoidal flow configuration (down to top) has been developed to maintain spheroids, located in the bottom chamber of LB2, in floating conditions.
Yes, the sigmoidal flow configuration (top to down) has been developed to perfuse a thick and porous scaffold, placed onto the membrane surface.
Protocols
MultiDyn
MultiDyn (MD) is a compact multi-dynamic-chambers rack, based on the dimensions of a standard 24-well plate.
Each rack is transparent and equipped with 4 wells, designed to reproduce the cell growth area of a standard plate. Each chamber can be autonomously perfused, allowing for the development of 4 parallel and dynamic models.
MD is an semi-disposable product and it can be provided with a clamp system and a plate, to increase the compatibility with LiveFlow.
FAQ
Yes. MD is desinged to reproduce both the bulk dimension as well as the cell growth surface/well of a standard 24-well plate. Therefore, both standard cell culture protocols as well as LiveBox1 protocols can be maintained.
Moreover, MD is trasparent, thus the cells can be monitored in real-time, using an inverted microscope.
Each rack is equipped with 4 wells. Each chamber can be autonomously perfused, allowing for the development of 4 parallel and dynamic models.
Yes, each rack is modular. Therefore, it is possible to join different MD to design, as in a Lego strategy approach, a customized fluidic platform, characterized by several tissues in cross-modulation and, at the same time, 4 replicates.
MD can be sterilized up to 3 times (we suggest: EtOH 70% + UV exposition).
